LOCUS       sequence-55996-e         355 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T
  TITLE     Arl13b regulates ciliogenesis and the dynamic localization of Shh 
            signaling proteins.
  JOURNAL   Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5.
  PUBMED    21976698
REFERENCE   2  (bases 1 to 355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2011-12"; volume: "22(23)"; pages: "4694-703. Epub 
            2011 Oct 5"
COMMENT     Sequence Label: pENTR-Arl13b2
FEATURES             Location/Qualifiers
     source          1..355
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     155..171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     155..171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     176..195
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        176..194
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    212..311
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact
       61 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
      121 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
      181 cgactcacta taggggatat cagctggatg gcaaataatg attttatttt gactgatagt
      241 gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa ctttgtacaa
      301 gaaagctggg tcggcgcgcc caccctttga gatcgtgtcc tgagcatcac cgtta
//
