LOCUS       sequence-55998-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR L1-13XLexAop2-R5
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     148..164
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     148..164
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     169..188
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        169..187
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(243..366)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(371..609)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(403..424)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
       61 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
      121 agattttgag acacgggcca gagctgccag gaaacagcta tgaccatgta atacgactca
      181 ctatagggga tatcgcggcc gccctgcagt acaggtcact aataccatct aagtagttga
      241 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      301 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttgtataca
      361 aagttgtatt cagagttctc ttcttgtatt caataattac ttcttggcag atttcagtag
      421 ttgcagttga tttacttggt tgctggttac ttttaattga ttcactttaa cttgcacttt
      481 actgcagatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg
      541 acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc gcctctattt
      601 atactccggc gctcgacgtc actagtgcta gagtctccgc ttactgtatg gatgtacagt
      661 acttactgta tggatgtaca gtacttactg tatggatgta cagtacttac tgtatggatg
      721 tacagtactt actgtatgga tgtacagtaa cctgcaggca tgcgctagcg ctag
//
