LOCUS       sequence-56001-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L1-10XQUAS-R5
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     149..165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     149..165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     170..189
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        170..188
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(244..367)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(404..642)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(436..457)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
       61 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
      121 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
      181 actatagggg atatcgcggc cgccctgcag tacaggtcac taataccatc taagtagttg
      241 attcatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
      301 atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac ttttgtatac
      361 aaagttgtct gagtccaacc cagtgagacc ccaattccct attcagagtt ctcttcttgt
      421 attcaataat tacttcttgg cagatttcag tagttgcagt tgatttactt ggttgctggt
      481 tacttttaat tgattcactt taacttgcac tttactgcag attgtttagc ttgttcagct
      541 gcgcttgttt atttgcttag ctttcgctta gcgacgtgtt cactttgctt gtttgaattg
      601 aattgtcgct ccgtagacga agcgcctcta tttatactcc ggcgctcgct agagtctccg
      661 ctcgggctgc agcaattcaa ggtcactgag tcccaacgtg aaaggataag cgattacccg
      721 aggataagcg attacccgag cggataagcg attacccgtg aggataattg tttatcc
//
