LOCUS       sequence-56121-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Parikh MR, Greene DN, Woods KK, Matsumura I
  TITLE     Directed evolution of RuBisCO hypermorphs through genetic selection 
            in engineered E.coli.
  JOURNAL   Protein Eng Des Sel. 2006 Mar;19(3):113-9. Epub 2006 Jan 19.
  PUBMED    16423843
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel."; date: "2006-03"; volume: "19(3)"; pages: "113-9. Epub 
            2006 Jan 19"
COMMENT     Sequence Label: rbcLS-pMAL-2px
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agctgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcctgcagg
      181 tcgacggagc tcaggcttta gtagcggccg ggacgatgaa cgatgaagct cacggtttgg
      241 cactgcttga tgttgtcgaa gccagcgaca cggatgtagc aatcaccgta ttcgctgcgg
      301 cactcacgca cttcatcgag gacttgctga gggctcttgc agtcaaacag ggggagcttc
      361 cacatcgtcc agtagaactc ttccggattc gagtgctcgt tgaactcgat caaggggtgg
      421 aagccttgct cgatcatgta ctcgatttgt gcagcgattt ggcgatcgct gaggggaggc
      481 aggtacgaga aagtctcgaa acgacgctct ttgggcagag ttttcatgct catgattcag
      541 aaatcctgaa aggttaagtc gaaaggctgg ggagaaagct ttacgcagca acgctcactc
      601 ccccagcgat agtcagaggc tccttagagc ttgtccatcg tttcgaattc gaacttgatc
      661 tctttccaga ggtcgagggc agcagccagt tcaggcgacc acttgccagc ttcacgaagg
      721 atgtcgccgc cttcacggta gaggtcgcga ccttcgttcc gagcttggac gcaagcttcc
      781 aaggcaacac ggttcgcggt tgcaccagga gcattaccc
//
