LOCUS       sequence-56122-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN beta
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagaa ttgccaccat ggactacaag gacgacgatg
       61 acaagaattc tgcaggatcc aagatggaat catctccatt taatagacgg caatggacct
      121 cactatcatt gagggtaaca gccaaagaac tttctcttgt caacaagaac aagtcatcgg
      181 ctattgtgga aatattctcc aagtaccaga aagcagctga agaaacaaac atggagaaga
      241 agagaagtaa caccgaaaat ctctcccagc actttagaaa ggggaccctg actgtgttaa
      301 agaagaagtg ggagaaccca gggctgggag cagagtctca cacagactct ctacggaaca
      361 gcagcactga gattaggcac agagcagacc atcctcctgc tgaagtgaca agccacgctg
      421 cttctggagc caaagctgac caagaagaac aaatccaccc cagatctaga ctcaggtcac
      481 ctcctgaagc cctcgttcag ggtcgatatc cccacatcaa ggacggtgag gatcttaaag
      541 accactcaac agaaagtaaa aaaatggaaa attgtctagg agaatccagg catgaagtag
      601 aaaaatcaga aatcagtgaa aacacagatg cttcgggcaa aatagagaaa tataatgttc
      661 cgctgaacag gcttaagatg atgtttgaga aaggtgaacc aactcaaact aagattctcc
      721 gggcccaaag ccgaagtgca agtggaagga agatctctga aaacagctat tctctagatg
      781 acctggaaat aggcccaggt cagttgtcat cttctacatt tgactcggag aaaaatgaga
      841 gtagacgaaa tctggaactt ccacgcctct cagaaacctc tataaaggat cgaatggcca
      901 agtaccaggc agctgtgtcc aaacaaagca gctcaaccaa ctatacaaat gagctgaaag
      961 ccagtggtgg cgaaatcaaa attcataaaa tggagcaaaa ggagaatgtg cccccaggtc
     1021 ctgaggtctg catcacccat caggaagggg aaaagatt
//
