LOCUS       sequence-56127-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pLR-NH
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     87..109
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     101..118
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     102..118
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaggctcagt cggaagactg ggcctttcgt tttatttgat gcctggcagt tccctactct
       61 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
      121 taagctcggg cccgtctcgc tatcgccagc aatcacggcg gcaagcaagc gctcgaaagc
      181 attgtggccc agctgagccg gcctgatccg gcgttggccg cgttgaccaa cgagagacgt
      241 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
      301 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
      361 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      421 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
      481 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      541 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
      601 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
      661 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
      721 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
      781 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
      841 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
      901 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
      961 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgaca
//
