LOCUS       sequence-56128-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN delta N-term
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgttagtgaa ccgtcagaat tgccaccatg gactacaagg acgacgatga
       61 caagaattct gcaggatcca agaagtttca ggcacctgca agagagacct gcgtggaatg
      121 tcagaagaca gtctatccaa tggagcgtct cttggccaac cagcaggtgt ttcacatcag
      181 ctgcttccgt tgctcctatt gcaacaacaa actcagtcta ggaacatatg catctttaca
      241 tggaagaatc tattgtaagc ctcacttcaa tcaactcttt aaatctaagg gcaactatga
      301 tgaaggcttt gggcacagac cacacaagga tctatgggca agcaaaaatg aaaacgaaga
      361 gattttggag agaccagccc agcttgcaaa tgcaagggag acccctcaca gcccaggggt
      421 agaagatgcc cctattgcta aggtgggtgt cctggctgca agtatggaag ccaaggcctc
      481 ctctcagcag gagaaggaag acaagccagc tgaaaccaag aagctgagga tcgcctggcc
      541 accccccact gaacttggaa gttcaggaag tgccttggag gaagggatca aaatgtcaaa
      601 gcccaaatgg cctcctgaag acgaaatcag caagcccgaa gttcctgagg atgtcgatct
      661 agatctgaag aagctaagac gatcttcttc actgaaggaa agaagccgcc cattcactgt
      721 agcagcttca tttcaaagca cctctgtcaa gagcccaaaa actgtgtccc cacctatcag
      781 gaaaggctgg agcatgtcag agcagagtga agagtctgtg ggtggaagag ttgcagaaag
      841 gaaacaagtg gaaaatgcca aggcttctaa gaagaatggg aatgtgggaa aaacaacctg
      901 gcaaaacaaa gaatctaaag gagagacagg gaagagaagt aaggaaggtc atagtttgga
      961 gatggagaat gagaatcttg tagaaaatgg tgcagactcc gatgaagatg ataacagctt
     1021 cctcaaacaa caatctccac aagaacccaa gtctctgaat tggtc
//
