LOCUS       sequence-56129-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA
  TITLE     The Polo kinase Plk4 functions in centriole duplication.
  JOURNAL   Nat Cell Biol. 2005 Nov;7(11):1140-6.
  PUBMED    16244668
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2005-11"; volume: "7(11)"; pages: "1140-6"
COMMENT     Sequence Label: pcDNA3 Plk4(Sak) D154A (Nigg HR26)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             149..178
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             182..211
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             215..238
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tagcaggagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagctta ccatggagca
      121 gaagctgatc tccgaggagg acctgaacga gcagaagctg atctccgagg aggacctgaa
      181 cgagcagaag ctgatctccg aggaggacct gaacctggag gtgctgttcc agggacccgg
      241 ggtacctagg atcctgacca tggcgacctg catcggggag aagatcgagg attttaaagt
      301 tggaaatctg cttggtaaag gatcatttgc tggtgtctac agagctgagt ccattcacac
      361 tggtttggaa gttgcaatca aaatgataga taagaaagcc atgtacaaag caggaatggt
      421 acagagagtc caaaatgagg tgaaaataca ttgccaattg aaacatccct ctatcttgga
      481 gctttataac tattttgaag atagcaatta tgtgtatctg gtattagaaa tgtgccataa
      541 tggagaaatg aacaggtatc taaagaatag agtgaaaccc ttctcagaaa atgaagctcg
      601 acacttcatg caccagatca tcacagggat gttgtatctt cattctcatg gtatactaca
      661 ccgggacctc acactttcta acctcctact gactcgtaat atgaacatca agattgctgc
      721 ttttgggctg gcaactcaac tgaaaatgcc acatgaaaag cactatacat tatgtggaac
      781 tcctaactac atttcaccag aaattgccac tcgaagtgca catggccttg aatctgatgt
      841 ttggt
//
