LOCUS       sequence-56130-e        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN alpha delta LIM
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagaa ttgccaccat ggactacaag gacgacgatg
       61 acaagaattc tgcaggatcc aaaatggaaa attgtctagg agaatccagg catgaagtag
      121 aaaaatcaga aatcagtgaa aacacagatg cttcgggcaa aatagagaaa tataatgttc
      181 cgctgaacag gcttaagatg atgtttgaga aaggtgaacc aactcaaact aagattctcc
      241 gggcccaaag ccgaagtgca agtggaagga agatctctga aaacagctat tctctagatg
      301 acctggaaat aggcccaggt cagttgtcat cttctacatt tgactcggag aaaaatgaga
      361 gtagacgaaa tctggaactt ccacgcctct cagaaacctc tataaaggat cgaatggcca
      421 agtaccaggc agctgtgtcc aaacaaagca gctcaaccaa ctatacaaat gagctgaaag
      481 ccagtggtgg cgaaatcaaa attcataaaa tggagcaaaa ggagaatgtg cccccaggtc
      541 ctgaggtctg catcacccat caggaagggg aaaagatttc tgcaaatgag aatagcctgg
      601 cagtccgttc cacccctgcc gaagatgact ccccaggtga ctcccaggtt aagagtgagg
      661 ttcaacagcc tgtccatccc aagccactaa gtccagattc cagagcctcc agtctttctg
      721 aaagttctcc tcccaaagca atgaagaagt ttcaggtacc tcacttcaat caactcttta
      781 aatctaaggg caactatgat gaaggctttg ggcacagacc acacaaggat ctatgggcaa
      841 gcaaaaatga aaacgaagag attttggaga gaccagccca gcttgcaaat gcaagggaga
      901 cccctcacag cccaggggta gaagatgccc ctattgctaa ggtgggtgtc ctggctgcaa
      961 gtatggaagc caaggcctcc tctcagcagg agaaggaaga cag
//
