LOCUS       sequence-56132-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN alpha delta N54
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagaa ttgccaccat ggactacaag gacgacgatg
       61 acaagaattc tgcaggatcc gcccaaagcc gaagtgcaag tggaaggaag atctctgaaa
      121 acagctattc tctagatgac ctggaaatag gcccaggtca gttgtcatct tctacatttg
      181 actcggagaa aaatgagagt agacgaaatc tggaacttcc acgcctctca gaaacctcta
      241 taaaggatcg aatggccaag taccaggcag ctgtgtccaa acaaagcagc tcaaccaact
      301 atacaaatga gctgaaagcc agtggtggcg aaatcaaaat tcataaaatg gagcaaaagg
      361 agaatgtgcc cccaggtcct gaggtctgca tcacccatca ggaaggggaa aagatttctg
      421 caaatgagaa tagcctggca gtccgttcca cccctgccga agatgactcc cgtgactccc
      481 aggttaagag tgaggttcaa cagcctgtcc atcccaagcc actaagtcca gattccagag
      541 cctccagtct ttctgaaagt tctcctccca aagcaatgaa gaagtttcag gcacctgcaa
      601 gagagacctg cgtggaatgt cagaagacag tctatccaat ggagcgtctc ttggccaacc
      661 agcaggtgtt tcacatcagc tgcttccgtt gctcctattg caacaacaaa ctcagtctag
      721 gaacatatgc atctttacat ggaagaatct attgtaagcc tcacttcaat caactcttta
      781 aatctaaggg caactatgat gaaggctttg ggcacagacc acacaaggat ctatgggcaa
      841 gcaaaaatga aaacgaagag attttggaga gaccagccca gcttgcaaat gcaagggaga
      901 cccctcacag cccaggggta gaagatgccc ctattgctaa ggtgggtgtc ctggctgcaa
      961 gtatggaagc caaggcctcc tctcagcagg agaaggaaga
//
