LOCUS       sequence-56133-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN alpha delta N112
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgttagtgac cgtcagaatt gccaccatgg actacaagga cgacgatgac
       61 aagaattctg caggatccgc caagtaccag gcagctgtgt ccaaacaaag cagctcaacc
      121 aactatacaa atgagctgaa agccagtggt ggcgaaatca aaattcataa aatggagcaa
      181 aaggagaatg tgcccccagg tcctgaggtc tgcatcaccc atcaggaagg ggaaaagatt
      241 tctgcaaatg agaatagcct ggcagtccgt tccacccctg ccgaagatga ctcccgtgac
      301 tcccaggtta agagtgaggt tcaacagcct gtccatccca agccactaag tccagattcc
      361 agagcctcca gtctttctga aagttctcct cccaaagcaa tgaagaagtt tcaggcacct
      421 gcaagagaga cctgcgtgga atgtcagaag acagtctatc caatggagcg tctcttggcc
      481 aaccagcagg tgtttcacat cagctgcttc cgttgctcct attgcaacaa caaactcagt
      541 ctaggaacat atgcatcttt acatggaaga atctattgta agcctcactt caatcaactc
      601 tttaaatcta agggcaacta tgatgaaggc tttgggcaca gaccacacaa ggatctatgg
      661 gcaagcaaaa atgaaaacga agagattttg gagagaccag cccagcttgc aaatgcaagg
      721 gagacccctc acagcccagg ggtagaagat gcccctattg ctaaggtggg tgtcctggct
      781 gcaagtatgg aagccaaggc ctcctctcag caggagaagg aagacaagcc agctgaaacc
      841 aagaagctga ggatcgcctg gccacccccc actgaacttg gaagttcagg aagtgccttg
      901 gaggaaggga tcaaaatgtc aaagcccaaa tggcctcctg aagacgaaat cagcaagccc
      961 gaagttcctg aggatgtcga tctagatctg aagaagctaa gacgatcttc ttc
//
