LOCUS       sequence-56135-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN alpha delta N173
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgttagtgac cgtcagaatt gccaccatgg actacaagga cgacgatgac
       61 aagaattctg caggatccgc agtccgttcc acccctgccg aagatgactc ccgtgactcc
      121 caggttaaga gtgaggttca acagcctgtc catcccaagc cactaagtcc agattccaga
      181 gcctccagtc tttctgaaag ttctcctccc aaagcaatga agaagtttca ggcacctgca
      241 agagagacct gcgtggaatg tcagaagaca gtctatccaa tggagcgtct cttggccaac
      301 cagcaggtgt ttcacatcag ctgcttccgt tgctcctatt gcaacaacaa actcagtcta
      361 ggaacatatg catctttaca tggaagaatc tattgtaagc ctcacttcaa tcaactcttt
      421 aaatctaagg gcaactatga tgaaggcttt gggcacagac cacacaagga tctatgggca
      481 agcaaaaatg aaaacgaaga gattttggag agaccagccc agcttgcaaa tgcaagggag
      541 acccctcaca gcccaggggt agaagatgcc cctattgcta aggtgggtgt cctggctgca
      601 agtatggaag ccaaggcctc ctctcagcag gagaaggaag acaagccagc tgaaaccaag
      661 aagctgagga tcgcctggcc accccccact gaacttggaa gttcaggaag tgccttggag
      721 gaagggatca aaatgtcaaa gcccaaatgg cctcctgaag acgaaatcag caagcccgaa
      781 gttcctgagg atgtcgatct agatctgaag aagctaagac gatcttcttc actgaaggaa
      841 agaagccgcc cattcactgt agcagcttca tttcaaagca cctctgtcaa gagcccaaaa
      901 actgtgtccc cacctatcag gaaaggctgg agcatgtcag agcagagtga agagtctgtg
      961 ggtggaagag ttgcagaaag gaaacaagtg gaaaatgcca aggcttcta
//
