LOCUS       sequence-56136-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN alpha delta domain 3
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgtttagtga ccgtcagaat tgccaccatg gactacaagg acgacgatga
       61 caagaattct gcaggatcca aaatggaaaa ttgtctagga gaatccaggc atgaagtaga
      121 aaaatcagaa atcagtgaaa acacagatgc ttcgggcaaa atagagaaat ataatgttcc
      181 gctgaacagg cttaagatga tgtttgagaa aggtgaacca actcaaacta agattctccg
      241 ggcccaaagc cgaagtgcaa gtggaaggaa gatctctgaa aacagctatt ctctagatga
      301 cctggaaata ggcccaggtc agttgtcatc ttctacattt gactcggaga aaaatgagag
      361 tagacgaaat ctggaacttc cacgcctctc agaaacctcc ggacctgagg tctgcatcac
      421 ccatcaggaa ggggaaaaga tttctgcaaa tgagaatagc ctggcagtcc gttccacccc
      481 tgccgaagat gactcccgtg actcccaggt taagagtgag gttcaacagc ctgtccatcc
      541 caagccacta agtccagatt ccagagcctc cagtctttct gaaagttctc ctcccaaagc
      601 aatgaagaag tttcaggcac ctgcaagaga gacctgcgtg gaatgtcaga agacagtcta
      661 tccaatggag cgtctcttgg ccaaccagca ggtgtttcac atcagctgct tccgttgctc
      721 ctattgcaac aacaaactca gtctaggaac atatgcatct ttacatggaa gaatctattg
      781 taagcctcac ttcaatcaac tctttaaatc taagggcaac tatgatgaag gctttgggca
      841 cagaccacac aaggatctat gggcaagcaa aaatgaaaac gaagagattt tggagagacc
      901 agcccagctt gcaaatgcaa gggagacccc tcacagccca ggggtagaag atgcccctat
      961 tgctaaggtg ggtgtcctgg ctgcaagtat ggaagccaag gcctcctctc
//
