LOCUS       sequence-56141-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Song Y, Maul RS, Gerbin CS, Chang DD
  TITLE     Inhibition of anchorage-independent growth of transformed NIH3T3 
            cells by epithelial protein lost in neoplasm (EPLIN) requires 
            localization of EPLIN to actin cytoskeleton.
  JOURNAL   Mol Biol Cell. 2002 Apr;13(4):1408-16.
  PUBMED    11950948
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-04"; volume: "13(4)"; pages: "1408-16"
COMMENT     Sequence Label: pCMV5-Flag-EPLIN beta delta LIM domain
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgtttagtga ccgtcagaat tgccaccatg gactacaagg acgacgatga
       61 caagaattct gcaggatcca agatggaatc atctccattt aatagacggc aatggacctc
      121 actatcattg agggtaacag ccaaagaact ttctcttgtc aacaagaaca agtcatcggc
      181 tattgtggaa atattctcca agtaccagaa agcagctgaa gaaacaaaca tggagaagaa
      241 gagaagtaac accgaaaatc tctcccagca ctttagaaag gggaccctga ctgtgttaaa
      301 gaagaagtgg gagaacccag ggctgggagc agagtctcac acagactctc tacggaacag
      361 cagcactgag attaggcaca gagcagacca tcctcctgct gaagtgacaa gccacgctgc
      421 ttctggagcc aaagctgacc aagaagaaca aatccacccc agatctagac tcaggtcacc
      481 tcctgaagcc ctcgttcagg gtcgatatcc ccacatcaag gacggtgagg atcttaaaga
      541 ccactcaaca gaaagtaaaa aaatggaaaa ttgtctagga gaatccaggc atgaagtaga
      601 aaaatcagaa atcagtgaaa acacagatgc ttcgggcaaa atagagaaat ataatgttcc
      661 gctgaacagg cttaagatga tgtttgagaa aggtgaacca actcaaacta agattctccg
      721 ggcccaaagc cgaagtgcaa gtggaaggaa gatctctgaa aacagctatt ctctagatga
      781 cctggaaata ggcccaggtc agttgtcatc ttctacattt gactcggaga aaaatgagag
      841 tagacgaaat ctggaacttc cacgcctctc agaaacctct ataaaggatc gaatggccaa
      901 gtaccaggca gctgtgtcca aacaaagcag ctcaaccaac tatacaaatg agctgaaagc
      961 cagtggtggc gaaatcaaaa ttcataaaat ggagcaaaag g
//
