LOCUS       sequence-56153-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_307
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             243..284
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    335..923
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(388..408)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(806..817)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttgata
      241 tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgaactagta
      301 ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
      361 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
      421 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
      481 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
      541 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
      601 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
      661 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
      721 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
      781 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
      841 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
      901 ctccctttgg gccgcctccc cgcgtcgact t
//
