LOCUS       sequence-56154-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX_307
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(19..229)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(460..504)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tctagaagct ttttgcaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata
       61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
      121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
      181 ctatggttgc tgactaattg agacgcatgc tttgcatact tctgcctgct ggggagcctg
      241 gggactttcc acacctggtt gctgactaat tgagatgcaa tgcatgggtc ccctcggggt
      301 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
      361 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
      421 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
      481 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
      541 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
      601 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
      661 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
      721 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
      781 ttgcgcctca atagccctca
//
