LOCUS       sequence-56155-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Maul RS, Sachi Gerbin C, Chang DD
  TITLE     Characterization of mouse epithelial protein lost in neoplasm 
            (EPLIN) and comparison of mammalian and zebrafish EPLIN.
  JOURNAL   Gene. 2001 Jan 10;262(1-2):155-60.
  PUBMED    11179679
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-01-10"; volume: "262(1-2)"; pages: "155-60"
COMMENT     Sequence Label: pCMV5-Flag-mEPLIN alpha
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             42..65
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(626..637)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tagcagagct cgtttagtga accgtcagaa ttgccaccat ggactacaag gacgacgatg
       61 acaagaattc tgcaggatcc aaaatggaaa actgtctggg agattccagg catgaagcag
      121 agaagccaga gacgagcgaa aacacagaaa cttcaggcaa aatagagaaa tacaacgttc
      181 cactgaatag actgaagatg atgtttgaga aaggtgaaca caaccaaacc aagagtctct
      241 ggacccaaaa ccgaaatgcg ggtggaagga ggctctctga aaacaactgt tccctggatg
      301 actgggaaat aggtgcgggt catttgtctt cctctgcatt caactcggag aaaaacgaga
      361 gtaagaggaa tctggagctg ccacgcctct cagaaacctc cataaaggac cgcatggcca
      421 agtaccaggc tgcagtgtcc aagcagagca gcccagccag ctacacaaat gagctgaaaa
      481 ccagtgaaag caaaactcat aaatgggaac agaaggagaa tgtgccccca ggtcccgagg
      541 cctgcagcgt ccatcaggaa ggaagcaagg tttctacaac tgagaatagt ctagtggccc
      601 tctctgtccc tgctgaagat gacacctgta actcccaggt gaagagcgag gcccagcagc
      661 ctatgcaccc taagccgctg agtcctgacg ccaggacctc cagtcttccg gaaagttctc
      721 cttccaaaac agcgaagaag tttcaggcgc cggcaaagga gagctgcgtg gagtgtcaga
      781 agacggtgta ccccatggaa cggctcctgg ccaaccagca ggtgtttcac atcagctgtt
      841 tccgatgctc ctactgcaac aacaagctca gtctaggaac atatgcatcc ttacatggac
      901 gaatctactg taagcctcac ttcaatcaac tcttcaagtc caaaggcaac tatgacgagg
      961 gctttgggca caagcagcat aaggatctgt gggcaagcaa gagcgacaat gaggagactt
     1021 tggggagac
//
