LOCUS       sequence-56158-e        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Maul RS, Sachi Gerbin C, Chang DD
  TITLE     Characterization of mouse epithelial protein lost in neoplasm 
            (EPLIN) and comparison of mammalian and zebrafish EPLIN.
  JOURNAL   Gene. 2001 Jan 10;262(1-2):155-60.
  PUBMED    11179679
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-01-10"; volume: "262(1-2)"; pages: "155-60"
COMMENT     Sequence Label: pCMV5-Flag-zEPLIN
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcagagct cgttagtgaa ccgtcagaat tgccaccatg gactacaagg acgacgatga
       61 caagaattcg atgtcagtga gttcatttcg gcgtggtcaa tgggcctccc agtcattgcg
      121 agtgacggca aaggagctct ccatagtcgg tgtgcggggc aaaaacacag ccattgctga
      181 gcgcttttcc aagtaccaga aggcagcaga agaaacaagt ttggacaaga aaaaatctcc
      241 ggagaaatcg actccaggcc ttcgcaacgg caatctgagt gtcctaaagc agctctggga
      301 acatccagcc gaaacgccta catctccaga acccaaagca cacctccaga accatctgca
      361 acagtctgct gtaaaaatcc cactggagtc cactgacgtc cagcttattg agggcacaga
      421 ccagcagtgt ttgagcgaca gtgatcagcc aatggagaag cgaacgcaaa gagatgtgga
      481 gaccctcatc gaaaagccca ccgtccccct caacagcctg aagatgatgt tcgagaaagg
      541 agagaccctt catagtaatg tgtccagaga gcctgggaca acaggagaca gtggatctga
      601 caatatggag ccaggaacta aagaaagtct ggagtgtggg gtaaaaatgc tggattcaac
      661 ccctctcagg gacaggatgg ccatgtacca ggcagctgtg actaagcttg atttcccatc
      721 ctcccccaat agtgaagcag cagacagtga agcccgagct cacagtggaa aacaaaagga
      781 aaatgtgcca ccagtgtctg ctgatgtgtg ccccgaatcc aacactatca aaagtcctgc
      841 tccagacagg aatggttctg ttttaagtcc tgagcaaaac cagcctaaat tggttaagat
      901 gttccctttg cctgtccgtg aaacctgcgt gacgtgcctt aagactgtgt acccactaga
      961 gaaacttgta gcgaatcagc agatttacca caacacctgt ttccgctgtg cctactgcaa
     1021 c
//
