LOCUS       sequence-56164-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  TITLE     Root Lab pLEX and pLIX Gateway backbones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX_403
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..53,54..89,90..92,93..128,129..131,
                     132..167,168..173,174..791))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 caacatagtt aagaatacga tatcttggat cgatccttac ttagttaccc ggggagcatg
       61 tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg
      121 gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt
      181 tcgcacttta gctgtttctc caggccacat atgattagtt ccaggccgaa aaggaaggca
      241 ggttcggctc cctgccggtc gaacagctca attgcttgtc tcagaagtgg gggcatagaa
      301 tcggtggtag gtgtctctct ttcctctttt gctacttgat gctcctgttc ctccaatacg
      361 cagcccagtg taaagtggcc cacggcggac agagcgtaca gtgcgttctc cagggagaag
      421 ccttgctgac acaggaacgc gagctgattt tccagggttt cgtactgttt ctctgttggg
      481 cgggtgccga gatgcacttt agccccgtcg cgatgtgaga ggagagcaca gcggtatgac
      541 ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt ccagggggca gaagtgggta
      601 tgatgcctgt ccagcatctc gattggcagg gcatcgagca gggcccgctt gttcttcacg
      661 tgccagtaca gggtaggctg ctcaactccc agcttttgag cgagtttcct tgtcgtcagg
      721 ccttcgatac cgacaccatt gagtaattcc agagctccgt ttatgacttt gctcttgtcc
      781 agtctagaca ttggaccagg gttttcttca acatcaccac aagtgaggag aga
//
