LOCUS       sequence-56167-e         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo single-strand annealing substrate composed of two truncated 
            GFP genes, separated by a drug selection marker, and flanked by 
            mouse hprt targeting sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Stark JM, Pierce AJ, Oh J, Pastink A, Jasin M
  TITLE     Genetic steps of mammalian homologous repair with distinct mutagenic
            consequences.
  JOURNAL   Mol Cell Biol. 2004 Nov;24(21):9305-16.
  PUBMED    15485900
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2004-11"; volume: "24(21)"; pages: "9305-16"
COMMENT     Sequence Label: hprtSAGFP
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(54..73)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
       61 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      121 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      181 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      241 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      301 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      361 agtccgccct gagcaaagac cccaacgaga agtgagaatt cactcctcag gtgcaggctg
      421 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
      481 tgatatcatc gatgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
      541 atgcgcttta aca
//
