LOCUS       sequence-56182-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR L1-13XLexAop2-L4
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        510..748
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     695..716
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(756..776)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctcttggcca gggccgcaag cttgcatgcc
      121 tgcaggttac tgtacatcca tacagtaagt actgtacatc catacagtaa gtactgtaca
      181 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagcgg
      241 agactctagc cctagggcat gcctgcaggt tactgtacat ccatacagta agtactgtac
      301 atccatacag taagtactgt acatccatac agtaagcgga gactctagcg ctagcgcatg
      361 cctgcaggtt actgtacatc catacagtaa gtactgtaca tccatacagt aagtactgta
      421 catccataca gtaagtactg tacatccata cagtaagtac tgtacatcca tacagtaagc
      481 ggagactcta gcactagtga cgtcgagcgc cggagtataa atagaggcgc ttcgtctacg
      541 gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa
      601 taaacaagcg cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc
      661 aattaaaagt aaccagcaac caagtaaatc aactgcaact actgaaatct gccaagaagt
      721 aattattgaa tacaagaaga gaactctgaa tcacccaact tttctataca aagttggcat
      781 tataagaaag cattgcttat caat
//
