LOCUS       sequence-56184-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L1-10XQUAS-R5
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        364..602
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     549..570
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    639..762
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagcagg ctctgctcac gtgtcactgg gtcagtgggt
      121 aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa
      181 tcgcttatcc tcgggtaatc gcttatcctt gggtaatcgc ttatcctcgg ataaacaatt
      241 atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt aatcgcttat
      301 cctttcacgt tgggactcag tgaccttgaa ttgctgcagc ccgagcggag actctagcga
      361 gcgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca
      421 aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa
      481 acaatctgca gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta
      541 aatcaactgc aactactgaa atctgccaag aagtaattat tgaatacaag aagagaactc
      601 tgaataggga attggggtct cactgggttg gactcagaca actttgtata caaaagttga
      661 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      721 gactacataa tactgtaaaa cacaacatat ccagtcacta tgaatcaact acttagatgg
      781 tattagtgac ctgtactgca gggcggccgc gatatcccct at
//
