LOCUS       sequence-56197-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: L1-10XQUAS-L4
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        365..603
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     550..571
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(643..738)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gctctgctca cgtgtcactg ggtcagtggg
      121 taatcgctta tcctcggata aacaattatc ctcacgggta atcgcttatc cgctcgggta
      181 atcgcttatc ctcgggtaat cgcttatcct tgggtaatcg cttatcctcg gataaacaat
      241 tatcctcacg ggtaatcgct tatccgctcg ggtaatcgct tatcctcggg taatcgctta
      301 tcctttcacg ttgggactca gtgaccttga attgctgcag cccgagcgga gactctagcg
      361 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
      421 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
      481 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt
      541 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact
      601 ctgaataggg aattggggtc tcactgggtt ggactcagca cccaactttt ctatacaaag
      661 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      721 aaaataaaat cattatttgc catccagctg cagggcggcc gc
//
