LOCUS       sequence-56216-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Jiang Z, Georgel P, Li C, Choe J, Crozat K, Rutschmann S, Du X, 
            Bigby T, Mudd S, Sovath S, Wilson IA, Olson A, Beutler B
  TITLE     Details of Toll-like receptor:adapter interaction revealed by 
            germ-line mutagenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):10961-6.
  PUBMED    16832055
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 18. 103(29):10961-6."
COMMENT     Sequence Label: pCMV-M2-TLR2-V660N
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..80
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             81..104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattaat tcaccatgtc tgcacttctg atcctagctc
       61 ttgttggagc tgcagttgct gactacaaag acgatgacga caagcttgcg gccgctcagg
      121 agtctctgtc atgtgatgct tctggggtgt gtgatggccg ctccaggtct ttcacctcta
      181 ttccctccgg actcacagca gccatgaaaa gccttgacct gtctttcaac aagatcacct
      241 acattggcca tggtgacctc cgagcgtgtg cgaacctcca ggttctgatt ttgaagtcca
      301 gcagaatcaa tacaatagag ggagacgcct tttattctct gggcagtctt gaacatttgg
      361 atttgtctga taatcaccta tctagtttat cttcctcctg gttcgggccc ctttcctctt
      421 tgaaatactt aaacttaatg ggaaatcctt accagacact gggggtaaca tcgctttttc
      481 ccaatctcac aaatttacaa accctcagga taggaaatgt agagactttc agtgagataa
      541 ggagaataga ttttgctggg ctgacttctc tcaatgaact tgaaattaag gcattaagtc
      601 tccggaatta tcagtcccaa agtctaaagt cgatccgcga catccattac ctgactcttc
      661 acttaagcga gtctgctttc ctgctggaga tttttgcaga tattctgagt tctgtgagat
      721 atttagaact aagagatact aacttggcca ggttccagtt ttcaccactg cccgtagatg
      781 aagtcagctc accgatgaag aagctggcat tccgaggctc ggttctcact gatgaaagct
      841 ttaacgagct cctgaagctg ttgcgttaca tcttggaact gtcggaggta gagttcgacg
      901 actgcaccct caatgggctc ggcgatttca acccctcgga gtcaga
//
