LOCUS       sequence-56219-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Stowers Lab Gateway MultiSite plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR L1-13XLexAop2-R5
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        509..747
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     694..715
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    752..772
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc tcttggccag ggccgcaagc ttgcatgcct
      121 gcaggttact gtacatccat acagtaagta ctgtacatcc atacagtaag tactgtacat
      181 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga
      241 gactctagcc ctagggcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca
      301 tccatacagt aagtactgta catccataca gtaagcggag actctagcgc tagcgcatgc
      361 ctgcaggtta ctgtacatcc atacagtaag tactgtacat ccatacagta agtactgtac
      421 atccatacag taagtactgt acatccatac agtaagtact gtacatccat acagtaagcg
      481 gagactctag cactagtgac gtcgagcgcc ggagtataaa tagaggcgct tcgtctacgg
      541 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
      601 aaacaagcgc agctgaacaa gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca
      661 attaaaagta accagcaacc aagtaaatca actgcaacta ctgaaatctg ccaagaagta
      721 attattgaat acaagaagag aactctgaat acaactttgt atacaaaagt tgaacgagaa
      781 acgtaaaatg atataaatat caatatatta aatta
//
