LOCUS       sequence-56229-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Ssb
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     238..255
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     269..288
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             347..370
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagcgactgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
      121 gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt
      181 ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      241 taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag
      301 gtccaactgc acctcggttc tatcgattga attcaccatg gaaatggact acaaggatga
      361 cgatgacaag ggtggtggtg gtggtgcgtc gacgatggct gaaaatggtg ataatgaaaa
      421 gatggctgcc ctggaggcca aaatctgtca tcaaattgag tattattttg gcgacttcaa
      481 tttgccacgg gacaagtttc taaaggaaca gataaaactg gatgaaggct gggtaccttt
      541 ggagataatg ataaaattca acaggttgaa ccgtctaaca acagacttta atgtaattgt
      601 ggaagcattg agcaaatcca aggcagaact catggaaatc agtgaagata aaactaaaat
      661 cagaaggtct ccaagcaaac ccctacctga agtgactgat gagtataaaa atgatgtaaa
      721 aaacagatct gtttatatta aaggcttccc aactgatgca actcttgatg acataaaaga
      781 atggttagaa gataaaggtc aagtactaaa tattcagatg agaagaacat tgcataaaag
      841 catttaaggg atcaattttt gttgtgtttg atagcattga atctgcta
//
