LOCUS       sequence-56233-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA
  TITLE     Sequence- and structure-specific RNA processing by a CRISPR 
            endonuclease.
  JOURNAL   Science. 2010 Sep 10;329(5997):1355-8.
  PUBMED    20829488
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-09-10"; volume: "329(5997)"; pages: "1355-8"
COMMENT     Sequence Label: pHMGWA-Pa14Csy4
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..85
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        66..84
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    85..109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             124..146
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             165..182
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatccagatc tcgatcccgc
       61 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
      121 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
      181 acggtaccaa aactgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata
      241 acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg
      301 agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc gatggccctg
      361 acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc ctgttggctg
      421 aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg gatgccgtac
      481 gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg ctgatttata
      541 acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag
      601 aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct
      661 ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca
      721 ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc
      781 tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta
      841 ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca
      901 gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca tcca
//
