LOCUS       sequence-56249-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Miller WG, Leveau JH, Lindow SE
  TITLE     Improved gfp and inaZ broad-host-range promoter-probe vectors.
  JOURNAL   Mol Plant Microbe Interact. 2000 Nov;13(11):1243-50.
  PUBMED    11059491
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact."; date: "2000-11"; volume: "13(11)"; pages: 
            "1243-50"
COMMENT     Sequence Label: pPROBE-gfp[tagless]
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(84..178)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(241..261)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     856..884
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gaaatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctgt
       61 aaaatctcgc caagctagct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
      121 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      181 agctcagcta attaagctta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      241 gttacaaact caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca
      301 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      361 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      421 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      481 tagttgtatt ccaacttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      541 aactcgattc tattaacaag tgtatcacct tcaaacttga cttcagcacg tgtcttatag
      601 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      661 ttgaaaaagt catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa
      721 gtcaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      781 ttaagggtaa gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg
      841 tgcccattaa catcaccatc taattcaaca agaattggga caactccagt
//
