LOCUS       sequence-56261-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNI_v2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(583..600)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(583..599)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(698..715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggtgtcgcc cttcgctgaa ctcaccccag agcaggtcgt ggcaattgcg agcaacatcg
       61 ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg
      121 gacgtcaaaa gggcgacaca aaatttattc taaatgcata ataaatactg ataacatctt
      181 atagtttgta ttatattttg tattatcgtt gacatgtata attttgatat caaaaactga
      241 ttttcccttt attattttcg agatttattt tcttaattct ctttaacaaa ctagaaatat
      301 tgtatataca aaaaatcata aataatagat gaatagttta attataggtg ttcatcaatc
      361 gaaaaagcaa cgtatcttat ttaaagtgcg ttgctttttt ctcatttata aggttaaata
      421 attctcatat atcaagcaaa gtgacaggcg cccttaaata ttctgacaaa tgctctttcc
      481 ctaaactccc cccataaaaa aacccgccga agcgggtttt tacgttattt gcggattaac
      541 gattactcgt tatcagaacc gcccaggggg cccgagctta agactggccg tcgttttaca
      601 acacagaaag agtttgtaga aacgcaaaaa ggccatccgt caggggcctt ctgcttagtt
      661 tgatgcctgg cagttcccta ctctcgcctt ccgcttcctc gctcactgac tcgctgcgct
      721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      901 acaaaaatcg acgctcaagt cagaggtggc gaaacccga
//
