LOCUS       sequence-56282-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNG_v2
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(589..606)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(589..605)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(704..721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attagggggt gtcgcccttc gctgaactca ccccagagca ggtcgtggca attgcgagca
       61 acggaggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag
      121 cgcacggacg tcaaaagggc gacacaaaat ttattctaaa tgcataataa atactgataa
      181 catcttatag tttgtattat attttgtatt atcgttgaca tgtataattt tgatatcaaa
      241 aactgatttt ccctttatta ttttcgagat ttattttctt aattctcttt aacaaactag
      301 aaatattgta tatacaaaaa atcataaata atagatgaat agtttaatta taggtgttca
      361 tcaatcgaaa aagcaacgta tcttatttaa agtgcgttgc ttttttctca tttataaggt
      421 taaataattc tcatatatca agcaaagtga caggcgccct taaatattct gacaaatgct
      481 ctttccctaa actcccccca taaaaaaacc cgccgaagcg ggtttttacg ttatttgcgg
      541 attaacgatt actcgttatc agaaccgccc agggggcccg agcttaagac tggccgtcgt
      601 tttacaacac agaaagagtt tgtagaaacg caaaaaggcc atccgtcagg ggccttctgc
      661 ttagtttgat gcctggcagt tccctactct cgccttccgc ttcctcgctc actgactcgc
      721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaa
//
