LOCUS       sequence-56299-e        3950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Martin DS, Fathi R, Mitchison TJ, Gelles J
  TITLE     FRET measurements of kinesin neck orientation reveal a structural 
            basis for processivity and asymmetry.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5453-8. Epub 2010 Mar 
            8.
  PUBMED    20212149
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2010-03-23"; volume: "107(12)"; pages: 
            "5453-8. Epub 2010 Mar 8"
COMMENT     Sequence Label: pDM8
FEATURES             Location/Qualifiers
     source          1..3950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1493..1510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1592..2383,2384..2452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2215..2234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2453..2557)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2625..2643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2773..3361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3262..3281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3515..3532
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3623..3642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3785..3807
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3872..3891
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3872..3890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3922..3944
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tatgtccgcg gaacgagaga ttcccgccga ggacagcatc aaagtggtct gccgattccg
       61 accgctgaac gacagcgaag agaaggccgg ctccaagttc gtggtcaagt tccccaacaa
      121 tgtggaggag aactccatat ccatagcggg caaggtgtat ttgttcgaca aggtcttcaa
      181 accgaatgca tcccaggaaa aggtctacaa tgaggcggcc aagtccattg ttacggatgt
      241 cctggccggg tacaatggaa cgatattcgc atatggtcag acgtcctccg gaaaaacgca
      301 tacgatggag ggcgtgatcg gggactccgt aaaacagggt atcataccac gtatcgtcaa
      361 cgacattttc aatcacatct acgcgatgga ggtgaacctg gagtttcaca tcaaggtctc
      421 ctactacgag atctacatgg acaagattcg agatctgttg gacgtctcca aggtgaacct
      481 tagtgtgcac gaggataaga accgggtgcc gtacgtcaag ggcgctacgg aacggttcgt
      541 ctcgtcgccg gaggatgttt tcgaggtgat cgaggagggc aaatccaatc gtcacatcgc
      601 tgtgacaaac atgaacgagc attcttcgcg atcccactca gtattcctta tcaatgtgaa
      661 gcaggagaac ctggagaacc agaagaaact atccggcaaa ctctacctgg tggatttggc
      721 cggttccgag aaggtttcca agactggagc ggagggaacc gttcttgatg aagccaagaa
      781 catcaacaag tcgctgtcgg ccttgggcaa cgtaatttct gccctggcgg acggaaacaa
      841 aacgcacatc ccctaccgtg attccaagct aacgcgcatc ctgcaggagt cgctgggagg
      901 caacgcacgc acaaccatcg tcatctgctg ctctccagcc agtttcaacg aatctgaaac
      961 gaagtcaacg ctggacttcg gtcgtagagc caagacagtg aagaacgtgg tctccgttaa
     1021 cgaggagctt actgccgagg aatggaagcg acgctatgaa aaggagaagg aaaagaacgc
     1081 ccgactaaag tgcaaggtgg agaagctgga gatcgagctt gcgcgctgga gagcgggtga
     1141 aactgttaag gcggaggagc aaatcaacat ggaggatctc atggaggcaa gcacgcccaa
     1201 cctgcgcaag gccatggaag cgccagcagc agcggaaatc agtggtcaca tcgtacgttc
     1261 cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt tcatcgaagt
     1321 gggtcagaaa gtcaacgtgg gcgataccct gtccatcgtt gaagccatga aaatgatgaa
     1381 ccagatcgaa gcggacaaat ccggtaccgt taaagctatc cttgttgaat ctggtcagcc
     1441 ggttgaattc gacgaaccgc ttgttgttat cgaattatct gagactagtg ggcaccacca
     1501 tcaccatcac tgacaagctt atcgatgata agctgtcaaa catgagaatt aaatcaatct
     1561 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1621 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1681 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1741 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1801 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1861 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1921 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1981 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2041 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2101 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2161 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     2221 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2281 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2341 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2401 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2461 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2521 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2581 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2641 ggccctttcg tcttcaagaa ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2881 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3421 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3481 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3541 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataca
     3601 gatctgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     3661 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     3721 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3781 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggccc
     3841 agcgattcga acttctgata gacttcgaaa ttaatacgac tcactatagg gagaccacaa
     3901 cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
//
