LOCUS       sequence-56326-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TALEN-ID12-C
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     376..395
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        470..574
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(793..812)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
       61 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
      121 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
      181 aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg
      241 caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg
      301 tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat
      361 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg
      421 gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      721 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      901 gcatcttacg gatggcatga cagtaagaga
//
