LOCUS       sequence-56329-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V
  TITLE     A ligation-independent cloning technique for high-throughput 
            assembly of transcription activator-like effector genes.
  JOURNAL   Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460.
  PUBMED    23242165
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460."
COMMENT     Sequence Label: TAL-1GT2
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..39
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(343..359)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(344..360)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(384..403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(413..430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(413..429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      571..929
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(658..677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     868..889
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
       61 cgcggtggcg gccgctctag cccgggcgga tcccccgggc tgcaggaatt cgatccaaat
      121 gaatgcagac acccgaccaa gttgtggcca ttgccagcaa caagggaggg aaacaagcat
      181 tggagactgt ccaacggctc cttcccgtgt tgtgtcaagc ccacggtttg acccctgcac
      241 aagtggtcgc catcgcctcc aacggtggcg gtaagcaggc cctggaaaca gtgcaacggt
      301 tgctccctgt cttgtgtctg cattcccaaa tatcaagctt atcgataccg tcgacctcga
      361 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
      421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      721 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      781 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      901 accctatctc ggtctattct tttgatttat a
//
