LOCUS       sequence-56330-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: IL2 pAcGP67a
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     124..183
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             592..609
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
       61 cgcggatcta tgctactagt aaatcagtca caccaaggct tcaataagga acacacaagc
      121 aagatggtaa gcgctattgt tttatatgtg cttttggcgg cggcggcgca ttctgccttt
      181 gcggcggatc cagcacctac ttcaagttct acaaagaaaa cacagctaca actggagcat
      241 ttacttctgg atttacagat gattttgaat ggaattaata attacaagaa tcccaaactc
      301 accaggatgc tcacatttaa gttttacatg cccaagaagg ccacagaact gaaacatctt
      361 cagtgtctag aagaagaact caaacctctg gaggaagtgc taaatttagc tcaaagcaaa
      421 aactttcact taagacccag ggacttaatc agcaatatca acgtaatagt tctggaacta
      481 aagggatctg aaacaacatt catgtgtgaa tatgctgatg agacagcaac cattgtagaa
      541 tttctgaaca gatggattac cttttgtcaa agcatcatct caacactaac tcatcatcac
      601 catcaccatt gagaattccg gagcggccgc tgcagatctg atcctttcct gggacccggc
      661 aagaaccaaa aactcactct cttcaaggaa atccgtaatg ttaaacccga cacgatgaag
      721 cttgtcgttg gatggaaagg aaaagagttc tacagggaaa cttggacccg cttcatggaa
      781 gacagcttcc ccattgttaa cgaccaagaa gtgatggatg ttttccttgt tgtcaacatg
      841 cgtcccacta gacccaaccg ttgttacaaa ttcctggccc aacacgctct gcgttgcgac
      901 cccgactatg tacctcatga cgtgattagg atcgtcgagc cttcatgggt gggcagca
//
