LOCUS       sequence-56331-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: IL2 F42A pAcGP67a
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        65..156
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     81..98
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     253..312
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             721..738
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgtaatgag acgcacaaac taatatcaca aactggaaat gtctatcaat atatagttgc
       61 tgatatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
      121 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
      181 accatcgggc gcggatctat gctactagta aatcagtcac accaaggctt caataaggaa
      241 cacacaagca agatggtaag cgctattgtt ttatatgtgc ttttggcggc ggcggcgcat
      301 tctgcctttg cggcggatcc agcacctact tcaagttcta caaagaaaac acagctacaa
      361 ctggagcatt tacttctgga tttacagatg attttgaatg gaattaataa ttacaagaat
      421 cccaaactca ccaggatgct cacagcaaag ttttacatgc ccaagaaggc cacagaactg
      481 aaacatcttc agtgtctaga agaagaactc aaacctctgg aggaagtgct aaatttagct
      541 caaagcaaaa actttcactt aagacccagg gacttaatca gcaatatcaa cgtaatagtt
      601 ctggaactaa agggatctga aacaacattc atgtgtgaat atgctgatga gacagcaacc
      661 attgtagaat ttctgaacag atggattacc ttttgtcaaa gcatcatctc aacactaact
      721 catcatcacc atcaccattg agaattccgg agcggccgct gcagatctg
//
