LOCUS       sequence-56333-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber
            ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC
  TITLE     Exploiting a natural conformational switch to engineer an 
            interleukin-2 'superkine'.
  JOURNAL   Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975.
  PUBMED    22446627
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-03-25- 25"; volume: "484"; issue: "7395"; 
            pages: "529-33"
COMMENT     Sequence Label: H9 F42A pAcGP67a
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..128
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     53..70
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     225..284
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     CDS             699..722
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             737..781
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             782..799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caaactggaa atgtctatca atatatagtt gctgatatca tggagataat taaaatgata
       61 accatctcgc aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac
      121 ctataaatat tccggattat tcataccgtc ccaccatcgg gcgcggatct atgctactag
      181 taaatcagtc acaccaaggc ttcaataagg aacacacaag caagatggta agcgctattg
      241 ttttatatgt gcttttggcg gcggcggcgc attctgcctt tgcggcggat cccgcaccta
      301 cttcaagttc tacaaagaaa acacagctac aactggagca tttacttctg gatttacaga
      361 tgattttgaa tggaattaat aattacaaga atcccaaact caccaggatg ctcacagcaa
      421 agttttacat gcccaagaag gccacagaac tgaaacatct tcagtgtcta gaagaagaac
      481 tcaaacctct ggaggaagtg ctaaatttag ctcagagcaa aaactttcac ttcgatccca
      541 gggacgtcgt cagcaatatc aacgtattcg tcctggaact aaagggatct gaaacaacat
      601 tcatgtgtga atatgctgat gagacagcaa ccattgtaga atttctgaac agatggatta
      661 ccttttgtca aagcatcatc tcaacactaa ctggcggcca tcatcaccac catcaccacc
      721 attaatgagc ggccgcggtc ttaatgatat tttcgaggct cagaagattg agtggcatga
      781 acatcatcat catcatcatt aaagatctg
//
