LOCUS       sequence-56346-e       10248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: pBOB-CAG-fHis-H2A-Ub
FEATURES             Location/Qualifiers
     source          1..10248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1674..1907
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2092..2136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2388..2505
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2515..2894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2897..3172
                     /label=chicken beta-actin promoter
     intron          3173..4181
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4105..4127
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             4257..4274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4275..4298
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4689..4916
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             4689..4916
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     misc_feature    5054..5642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5107..5127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5525..5536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6049..6229
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6255..6272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6261..6485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6531..6959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6618..6637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6828..6849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6968..6988)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6973..7303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7154..7289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7216..7235
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7351..7398
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7417..7791
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7921..8042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7958..7977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8012..8031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8091..8107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8091..8107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8104..8126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8115..8131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8139..8145,8146..8163,8164..8169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8184..8205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8322..8339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8493..9081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8573..8592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9252..10043,10044..10112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9875..9894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10113..10217)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccgctgatc ttcagacttg gaggaggaga tatgagggac
     1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc aatgacgctg
     1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccaa
     1861 gcaagaatcc tagctgtgga aagataccta aaggatcaac agctcctagg gatttggggt
     1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1981 tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2281 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt taaaagaaaa
     2401 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2461 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tgtcgacatt
     2521 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2581 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2641 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2701 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     2761 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2821 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2881 tcgctattac catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2941 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     3001 gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     3061 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     3121 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
     3181 tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg
     3241 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     3301 ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg
     3361 gctccgggag ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
     3421 gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     3481 cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg
     3541 cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     3601 gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga
     3661 gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc
     3721 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     3781 gccggggagg gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg
     3841 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     3901 tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     3961 cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     4021 ccgcgccgcc gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc
     4081 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     4141 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     4201 ttgttgtgct gtctcatcat tttggcaaag aattcgctag tggatccacc atggtgcatc
     4261 atcatcatca tcacgattac aaggatgacg acgataagat gtctgggcgt ggtaagcagg
     4321 gtggcaaggc gcgcgccaag gctaagtcgc gctcgtcgcg cgcggggctg cagttccccg
     4381 tgggccgcgt gcaccggttg ctccgcaagg gcaactattc ggagcgcgtg ggcgccggcg
     4441 ccccggtcta tctggccgcg gtgctcgagt acttgactgc cgagatcctg gagcttgccg
     4501 gcaacgcggc gcgcgacaac aagaagacgc gcatcatccc gcgccacctg cagctggcca
     4561 tccgcaacga cgaggagctc aacaagctgc tgggccgcgt gaccatcgcg cagggtggcg
     4621 tcctgcccaa catccaggcc gtgctgctgc caaagaaaac tgagagccac cacaaggcga
     4681 agggcaagat gcagatcttc gtgaagaccc tgactggtaa gaccatcact ctcgaagtgg
     4741 agccgagtga caccattgag aatgtcaagg caaagatcca agacaaggaa ggcatccctc
     4801 ctgaccagca gaggttgatc tttgctggga aacagctgga agatggacgc accctgtctg
     4861 actacaacat ccagaaagag tccaccctgc acctggtcct ccgtctcaga ggtgggtaag
     4921 ataacgtctc gagtttaaac ctggaaaaac atggagcaat cacaagtagc aacacagcag
     4981 ctaccaatgc tgcttgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     5041 tcacacctca gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5101 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5161 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5221 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5281 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5341 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5401 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5461 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5521 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5581 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5641 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5701 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagacaa
     5761 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg acagaactac
     5821 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5881 gttgagccag ataagataga agaggccaat aaaggagaga acaccagctt gttacaccct
     5941 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     6001 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg actgtactgg gtctctctgg
     6061 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6121 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6181 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggccgcttta
     6241 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     6301 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     6361 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     6421 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     6481 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     6541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6661 ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg
     6721 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6781 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6841 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6901 ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     6961 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag
     7021 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7321 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7381 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7441 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7501 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7561 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7621 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7681 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7741 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     7801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8041 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8101 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8161 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8221 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8281 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8341 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8401 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8461 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8521 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8581 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8641 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     8701 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8761 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8821 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8881 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8941 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9001 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9061 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9121 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9181 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9241 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9301 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9361 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9421 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9481 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9541 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9601 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9661 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9721 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9781 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9841 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9901 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9961 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10021 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10081 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10201 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
