LOCUS       sequence-56353-e       11420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: p156RRL-EF1a-GFP-U3H1SatA
FEATURES             Location/Qualifiers
     source          1..11420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2122..3299
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2353..3290
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3247..3267
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3347..3464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3548..3557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3554..3556,3557..3559,3560..4270)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3554..3556,3557..3559,3560..4270)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3599..3620)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3860..3879)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4207..4228
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4343..4931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4396..4416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4814..4825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5099..5117,5118..5140,5141..5159,5160..5182,5183..5201,
                     5202..5224,5225..5243,5244..5266,5267..5285,5286..5308,
                     5309..5327,5328..5350,5351..5369)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5398..5612
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5533..5552
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5982..6015)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6035..6215
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6240..6258)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6241..6258)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6548..6567)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6781..6800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6900..6922
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6960..6978)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7046..7150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7151..7219,7220..8011)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7369..7388)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8182..8770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8671..8690
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8924..8941
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9011..9031)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9016..9345
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9196..9331
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10479..10544
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10674..10694
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11119..11253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11156..11175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11210..11229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgcgatgtcg acgataagct
     2041 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     2101 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     2161 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     2221 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     2281 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     2341 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     2401 ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt gatcccgagc
     2461 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     2521 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     2581 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     2641 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     2701 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     2761 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     2821 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     2881 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     2941 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     3001 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     3061 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     3121 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     3181 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     3241 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     3301 gaatttcgac atttaaattt aattaatctc gacggtatcg gttaactttt aaaagaaaag
     3361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttccgatc acgagactag
     3481 cctcgaggtt taaactacgg gatcttcgaa ggcctaagct tacgcgcgcg tcctagcgct
     3541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3841 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3901 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3961 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4021 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4081 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4141 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4201 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4261 gctgtacaag tccggactca gatctcgata gctagtagct agctagctag tcgagctcaa
     4321 cttcggggac tagtcatatg ataatcaacc tctggattac aaaatttgtg aaagattgac
     4381 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     4441 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     4501 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     4561 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     4621 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     4681 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     4741 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     4801 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     4861 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     4921 cgcctccccg catcggtacg tatggccagg tacctttaag accaatgact tacaaggcag
     4981 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5041 aacgaagaca agatgggatc aattcaccat gctagtggat ccccctcgag tttaccactc
     5101 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     5161 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     5221 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     5281 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5341 agtttaccac tccctatcag tgatagagaa aagtgaaagt cggggctgca ggaattcgaa
     5401 cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac
     5461 ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc
     5521 aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat
     5581 ttgggaatct tataagttct gtatgagacc acgcgttctg tctagttttc atatgaagat
     5641 atcccgtgtc caacgaaatc ctcaaaggta tcaaaatatc cacttgcaga ttctacaaaa
     5701 agagtgcttc aaaactgctc tgtcaaaagg aaggttcaac tctgttactt gagtacacac
     5761 atcacaagga agtttctgag aatgctgcta gatctgtcta gttttcatat gaagatatcc
     5821 cgtgtccaac gaaatcctca aaggtatcaa aatatccact tgcagattct acaaaaagat
     5881 gtttccaaac tgctgtatca atagacaggt tgtactctgt tagttgagga cacacatcaa
     5941 aaagaagttt ctgagaatgc tatcgatacc gtcgcatggg aataacttcg tatagcatac
     6001 attatacgaa gttatgctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6061 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6121 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6181 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     6241 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     6301 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     6361 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     6421 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     6481 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     6541 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     6601 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     6661 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     6721 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     6781 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     6841 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6901 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     6961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7141 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7201 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7261 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7321 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7381 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7441 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7501 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7561 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7621 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7681 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7741 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7801 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7861 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7921 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7981 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8041 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8101 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     8341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     8581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8821 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8881 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8941 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9001 atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     9061 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     9121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     9181 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9301 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag
     9361 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     9421 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     9481 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     9541 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     9601 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     9661 tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     9721 atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc
     9781 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     9841 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     9901 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     9961 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
    10021 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
    10081 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
    10141 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
    10201 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
    10261 gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag
    10321 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa
    10381 ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat
    10441 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
    10501 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
    10561 ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag
    10621 aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa
    10681 agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc
    10741 atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag
    10801 ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata
    10861 acagttataa tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta
    10921 ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata
    10981 aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta
    11041 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    11101 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    11161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    11221 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    11281 catcccaaac ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt
    11341 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    11401 atgtactaca aacttagtag
//
