LOCUS       sequence-56366-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-TRP1
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..197
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             198..872
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaaggcgc gccagatctg tacaatcttg
       61 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
      121 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
      181 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
      241 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
      301 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
      361 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
      421 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
      481 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
      541 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
      601 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
      661 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
      721 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
      781 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
      841 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
      901 attgtttgtg cacttgccga tctatgcggt gtgaaatac
//
