LOCUS       sequence-56372-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..437
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             832..846
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa ggcgcgccag atctgtttag
       61 cttgcctcgt ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt
      121 gacagtcttg acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc
      181 atacatcccc atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc
      241 aaaaattacg gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg
      301 ttgaattgtc cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg
      361 aggttcttct ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca
      421 tccgaacata aacaaccatg gcagaaccag cccaaaaaaa gcaaaaacaa actgttcagg
      481 agcgcaaggc gtttatctcc cgtatcacta atgaaactaa aattcaaatc gctatttcgc
      541 tgaatggtgg ttatattcaa ataaaagatt cgattcttcc tgcaaagaag gatgacgatg
      601 tagcttccca agctactcag tcacaggtca tcgatattca cacaggtgtt ggctttttgg
      661 atcatatgat ccatgcgttg gcaaaacact ctggttggtc tcttattgtt gaatgtattg
      721 gtgacctgca cattgacgat caccatacta ccgaagattg cggtatcgca ttagggcaag
      781 cgttcaaaga agcaatgggt gctgtccgtg gtgtaaaaag attcggtact gggttcgcac
      841 cattggatga ggcgctatca cgtgccgtag tcgatttatc tagtagacca tttgctgtaa
      901 tcgaccttgg attgaagaga gagatgattg gtgatttatc cactgaaatg attc
//
