LOCUS       sequence-56376-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-His3MX6
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(69..266)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(270..968)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
ORIGIN
        1 ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg ttagtatcga
       61 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      121 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
      181 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
      241 aaaacaagaa tctttttatt gtcagtactt cacatcaaaa cacctttggt tgagggaacg
      301 tcattggtgc cattgctaga aatagcttct cttatggcaa cagccaaagc cttgaacgca
      361 ctctcacttc tgtggtgatc gttgaaacct ctcagacaat caacatgcaa agtaattctg
      421 gccgcctccg cgaaactttc caaaaagtgt ggaatcattt cagtggataa atcaccaatc
      481 atctctctct tcaatccaag gtcgattaca gcaaatggtc tactagataa atcgactacg
      541 gcacgtgata gcgcctcatc caatggtgcg aacccagtac cgaatctttt tacaccacgg
      601 acagcaccca ttgcttcttt gaacgcttgc cctaatgcga taccgcaatc ttcggtagta
      661 tggtgatcgt caatgtgcag gtcaccaata cattcaacaa taagagacca accagagtgt
      721 tttgccaacg catggatcat atgatccaaa aagccaacac ctgtgtgaat atcgatgacc
      781 tgtgactgag tagcttggga agctacatcg tcatccttct ttgcaggaag aatcgaatct
      841 tttatttgaa tataaccacc attcagcgaa atagcgattt gaattttagt ttcattagtg
      901 atacgggaga taaacgcctt gcgctcctga acagtttgtt tttgcttttt ttgggctggt
      961 tctgccatgg ttgtttatgt tcgga
//
