LOCUS       sequence-56396-e         969 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GFP(S65T)-His3MX6
FEATURES             Location/Qualifiers
     source          1..969
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..745
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(62..90)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     685..705
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      769..956
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(824..848)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacagtaaa ggagaagaac ttttcactgg
       61 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      121 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      181 tggaaaacta cctgttccat ggccaacact tgtcactact ttcacttatg gtgttcaatg
      241 cttttcaaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      301 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
      361 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
      421 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
      481 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
      541 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
      601 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
      661 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
      721 acatggcatg gatgaactat acaaataggg cgcgccactt ctaaataagc gaatttctta
      781 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
      841 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
      901 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
      961 tccgctagg
//
