LOCUS       sequence-56402-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-3HA-TRP1
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..135
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      168..355
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(223..247)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        391..538
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaaca tcttttaccc atacgatgtt
       61 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
      121 gttccagatt acgctgctca gtgctgaggc gcgccacttc taaataagcg aatttcttat
      181 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
      241 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
      301 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
      361 ccgctaggga taacagggta atatagatct gtacaatctt gatccggagc ttttcttttt
      421 ttgccgatta agaattaatt cggtcgaaaa aagaaaagga gagggccaag agggagggca
      481 ttggtgacta ttgagcacgt gagtatacgt gattaagcac acaaaggcag cttggagtat
      541 gtctgttatt aatttcacag gtagttctgg tccattggtg aaagtttgcg gcttgcagag
      601 cacagaggcc gcagaatgtg ctctagattc cgatgctgac ttgctgggta ttatatgtgt
      661 gcccaataga aagagaacaa ttgacccggt tattgcaagg aaaatttcaa gtcttgtaaa
      721 agcatataaa aatagttcag gcactccgaa atacttggtt ggcgtgtttc gtaatcaacc
      781 taaggaggat gttttggctc tggtcaatga ttacggcatt gatatcgtcc aactgcatgg
      841 agatgagtcg tggcaagaat accaagagtt cctcggtttg ccagttatta aaagactcgt
      901 at
//
