LOCUS       sequence-56406-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-3HA-His3MX6
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..135
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      168..355
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(223..247)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        430..773
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 tcgtacgctg caggtcgacg gatccccggg ttaattaaca tcttttaccc atacgatgtt
       61 cctgactatg cgggctatcc ctatgacgtc ccggactatg caggatccta tccatatgac
      121 gttccagatt acgctgctca gtgctgaggc gcgccacttc taaataagcg aatttcttat
      181 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
      241 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
      301 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
      361 ccgctaggga taacagggta atatagatct gtttagcttg cctcgtcccc gccgggtcac
      421 ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca
      481 ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt
      541 gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag
      601 acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc
      661 ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct
      721 tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca accatggcag
      781 aaccagccca aaaaaagcaa aaacaaactg ttcaggagcg caaggcgttt atctcccgta
      841 tcactaatga aactaaaatt caaatcgcta tttcgctgaa tggtggttat attcaaataa
      901 aagattcgat tcttcctgca aagaaggatg acgatgta
//
