LOCUS       sequence-56413-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-3HA-His3MX6
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(76..273)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(277..975)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
ORIGIN
        1 cactagtgga tctgatatca tcgatgaatt cgagctcgtt taaactggat ggcggcgtta
       61 gtatcgaatc gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac
      121 tttctgcgca cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat
      181 cacgctaaca tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa
      241 gttcttgaaa acaagaatct ttttattgtc agtacttcac atcaaaacac ctttggttga
      301 gggaacgtca ttggtgccat tgctagaaat agcttctctt atggcaacag ccaaagcctt
      361 gaacgcactc tcacttctgt ggtgatcgtt gaaacctctc agacaatcaa catgcaaagt
      421 aattctggcc gcctccgcga aactttccaa aaagtgtgga atcatttcag tggataaatc
      481 accaatcatc tctctcttca atccaaggtc gattacagca aatggtctac tagataaatc
      541 gactacggca cgtgatagcg cctcatccaa tggtgcgaac ccagtaccga atctttttac
      601 accacggaca gcacccattg cttctttgaa cgcttgccct aatgcgatac cgcaatcttc
      661 ggtagtatgg tgatcgtcaa tgtgcaggtc accaatacat tcaacaataa gagaccaacc
      721 agagtgtttt gccaacgcat ggatcatatg atccaaaaag ccaacacctg tgtgaatatc
      781 gatgacctgt gactgagtag cttgggaagc tacatcgtca tccttctttg caggaagaat
      841 cgaatctttt atttgaatat aaccaccatt cagcgaaata gcgatttgaa ttttagtttc
      901 attagtgata cgggagataa acgccttgcg ctcctgaaca gtttgttttt gctttttttg
      961 ggctggttct gccatggttg ttt
//
