LOCUS       sequence-56414-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-13Myc-TRP1
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..535
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      590..777
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(645..669)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgctgcaggt cgacggatcc ccgggttaat taacggtgaa caaaagctaa tctccgagga
       61 agacttgaac ggtgaacaaa aattaatctc agaagaagac ttgaacggac tcgacggtga
      121 acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct ccgaggaaga
      181 cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg acggtgaaca
      241 aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg aggaagactt
      301 gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg gtgaacaaaa
      361 gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg aagacttgaa
      421 cggtgaacaa aaattaatct cagaagaaga cttgaacgga ctcgacggtg aacaaaagtt
      481 gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag acttgaacgg
      541 tgaacaaaaa ttaatcaatc actagtgaat tcgcgccact tctaaataag cgaatttctt
      601 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
      661 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
      721 caggttgctt tctcaggtat agtatgaggt cgctcttatt gaccacacct ctaccggcag
      781 atccgctagg gataacaggg taatatagat ctgtacaatc ttgatccgga gcttttcttt
      841 ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg
      901 cattggtgac tattgagcac gtgagtatac gtgattaagc acaca
//
