LOCUS       sequence-56415-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Park TJ, Mitchell BJ, Abitua PB, Kintner C, Wallingford JB
  TITLE     Dishevelled controls apical docking and planar polarization of basal
            bodies in ciliated epithelial cells.
  JOURNAL   Nat Genet. 2008 Jul . 40(7):871-9.
  PUBMED    18552847
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2008 Jul . 40(7):871-9."
COMMENT     Sequence Label: pCS2+ tecktin-GFP
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             105..818
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(135..163)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     758..778
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc taccatgagt aaaggagaag
      121 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      181 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      241 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actctcactt
      301 atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac tttttcaaga
      361 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      421 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      481 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      541 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      601 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      661 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      721 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      781 ctgctgggat tacacatggc atggatgaac tatacaaagg atcagctgca ggatatcgag
      841 ctcggatccc atcgattcga attcaaggcc tctcgagttc agatatggcc actttga
//
