LOCUS       sequence-56418-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-13Myc-TRP1
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(56..203)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     terminator      complement(239..426)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     347..371
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(481..978)
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
ORIGIN
        1 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
       61 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
      121 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttaatcgg caaaaaaaga
      181 aaagctccgg atcaagattg tacagatcta tattaccctg ttatccctag cggatctgcc
      241 ggtagaggtg tggtcaataa gagcgacctc atactatacc tgagaaagca acctgaccta
      301 caggaaagag ttactcaaga ataagaattt tcgttttaaa acctaagagt cactttaaaa
      361 tttgtataca cttatttttt ttataactta tttaataata aaaatcataa atcataagaa
      421 attcgcttat ttagaagtgg cgcgaattca ctagtgattg attaattttt gttcaccgtt
      481 caagtcttcc tcggagatta gcttttgttc accgttcaaa tcttcttcag aaatcaactt
      541 ttgttcaccg tcgagtccgt tcaagtcttc ttctgagatt aatttttgtt caccgttcaa
      601 gtcttcctcg gagattagct tttgttcacc gttcaaatct tcttcagaaa tcaacttttg
      661 ttcaccgtcg agtccgttca agtcttcttc tgagattaat ttttgttcac cgttcaagtc
      721 ttcctcggag attagctttt gttcaccgtt caaatcttct tcagaaatca acttttgttc
      781 accgtcgagt ccgttcaagt cttcttctga gattaatttt tgttcaccgt tcaagtcttc
      841 ctcggagatt agcttttgtt caccgttcaa atcttcttca gaaatcaact tttgttcacc
      901 gtcgagtccg ttcaagtctt cttctgagat taatttttgt tcaccgttca agtcttcctc
      961 ggagattagc ttttgttcac cgttaattaa cccggggatc cgtcgacctg cagcgt
//
