LOCUS       sequence-56428-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-GST-TRP1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      44..231
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(99..123)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        267..414
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 aatcggatct ggttccgcgt tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt
       61 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
      121 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
      181 gctttctcag gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc
      241 tagggataac agggtaatat agatctgtac aatcttgatc cggagctttt ctttttttgc
      301 cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg agggcattgg
      361 tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg gagtatgtct
      421 gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt gcagagcaca
      481 gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat atgtgtgccc
      541 aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct tgtaaaagca
      601 tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa tcaacctaag
      661 gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact gcatggagat
      721 gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag actcgtattt
      781 ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc gtttattccc
      841 ttgtttgatt cagaagcagg tgggacaggt gaacttttgg attggaactc gatttctgac
      901 tgggttggaa ggcaagagag ccccgaaagc ttacatttta tgttagctgg tggactgacg
      961 ccagaa
//
