LOCUS       sequence-56435-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat 
            A, Philippsen P, Pringle JR
  TITLE     Additional modules for versatile and economical PCR-based gene 
            deletion and modification in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 1998 Jul;14(10):953-61.
  PUBMED    9717241
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "1998-07"; volume: "14(10)"; pages: "953-61"
COMMENT     Sequence Label: pFA6a-kanMX6-PGAL1
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(55..496)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(61..84)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    379..496
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gcaggtcgac ggatccccgg gttaattaaa gacattttga gatccgggtt ttttctcctt
       61 gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat
      121 aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg
      181 catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa
      241 taaggctaaa aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg
      301 aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat
      361 tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc
      421 gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg tcacccgctc
      481 ggcggcttct aatccgtact tcaatatagc aatgagcagt taagcgtatt actgaaagtt
      541 ccaaagagaa ggttttttta ggctaagata atggggctct ttacagatct gtttagcttg
      601 cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca
      661 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
      721 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
      781 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
      841 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
      901 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatcc
//
